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KMID : 0545120120220091253
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 9 p.1253 ~ p.1257
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Functional Expression of Amylosucrase, a Glucan-Synthesizing Enzyme, from Arthrobacter chlorophenolicus A6
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Seo Dong-Ho
Jung Jong-Hyun
Choi Hyun-Chang
Cho Hyun-Kuk
Kim Hee-Hang
Ha Suk-Jin
Yoo Sang-Ho
Cha Jae-Ho
Park Cheon-Seok
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Abstract
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A gene (acas) designated as α-amylase was cloned from Arthrobacter chlorophenolicus A6. The multiple amino acid sequence analysis and functional expression of acas revealed that this gene really encoded an amylosucrase (ASase) instead of α-amylase. In fact, the recombinant enzyme exhibited typical ASase activity by showing both sucrose hydrolysis and glucosyltransferase activities. The purified enzyme has a molecular mass of 72 kDa and exhibits optimal hydrolysis activity at 45oC and a pH of 8.0. The analysis of the oligomeric state of ACAS with gel permeation chromatography revealed that the ACAS existed as a monomer.
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KEYWORD
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amylosucrase, Arthrobacter chlorophenolicus, glucan-synthesizing enzyme, sucrose
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